The initial annealing temperature is set higher than the optimal melting temperature (Tm) of the primers and is then gradually reduced over subsequent cycles until the Tm or touchdown temperature is reached, much like the touchdown of an. No right is conveyed expressly, by implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits or methods such as 5’ nuclease methods. We have partnered with Amazon Web Services (AWS) to offer a robust platform. 2720 Thermal Cycler Precise control in a small footprint Reaction volume 1 L. Touchdown PCR is a modification of PCR used to help reduce non-specific annealing and amplification. The GeneAmp® PCR System 9700 thermal cycler is covered by US patent claims and corresponding claims in their non-US counterparts. It’s easy, convenient, and protected by the highest levels of privacy standards.įor peace of mind, the Thermo Fisher Cloud has high industry-standard security, so your work remains secure. Share your protocols or other files with other Thermo Fisher Connect users on campus and around the world. After each run, the run report will be conveniently saved in your account.Ĭheck run status and instrument availability in real-timeĭownload our Instrument Connect remote monitoring app to:ĭiscretionary access to project folders and protocols. A touchdown PCR program was implemented as follows: initial denaturation at 95C.
2720 thermal cycler touchdown pcr free#
Once your run is prepared, start it from your instrument or remotely with Thermo Fisher Connect. Ask for a free sample of our PCR plates to check compatibility with your thermal cycler (with no obligation. PCR was performed in an Applied Biosystem Thermal Cycler (Model 2720, USA). PCR reactions were performed in a 2720 Thermal Cycler (Applied Biosystems) using condi- tions optimised for each primer pairs.
touchdown reaction was performed in an Applied Biosystems 2720 Thermal Cycler. Access tips and tricks related to programming and running of your PCR machine, including ProFlex, Veriti, 2720, GeneAmp PCR System 9700, and SimpliAmp PCR thermal cyclers. Design protocols from anywhere easily maintain run reports.ĭevelop protocols on any desktop computer or mobile device and store them in your secure personal cloud account. Applied Biosystems Applied Biosystems 2720 Thermal Cycler Ideal for both basic PCR and cycle sequencing applications using 0. Microsatellite markers are highly variable and locus-specific PCR-based.
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